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The Effects of Cardiac Patch Material on Mesenchymal Stem Cells and Cardioprogenitor Cells
Russell Witt1; Chani Hodonsky2; Lakshimi Mundada2; Shunyun Wang2; Gary Raff3; Sunjay Kaushal4; Ming-Sing Si2
1 Brigham and Women's Hospital, Boston, MA; 2 University of Michigan, Ann Arbor, MI; 3 University of California at Davis Medical Center, Sacramento, CA; 4 University Maryland, Baltimore, MD,

Background: Polytetrafluoroethylene (PTFE) and porcine small intestinal submucosa (pSIS) are patch materials used in congenital heart surgery. Porcine SIS is an extracellularmatrix scaffold that may interact with stem/progenitor cells. To evaluate this, we determined the in vitro effects of pSIS and PTFE on human bone marrow mesenchymal stromal cells (MSCs) and cardioprogenitor cells (CPCs) in three areas: proliferation, differentiation, and angiogenic growth-factor production.

Methods:Human MSCs and CPCs were seeded onto pSIS and PTFE scaffolds. These were assessed for cell viability/proliferation and VEGFA levels. PCR was used to determine changes in gene expression related to angiogenesis and cardiogenesis.

Results: MSCs and CPCs attached to pSIS and PTFE. The proliferation rate of each cell type was significantly greater on pSIS. Total RNA isolation was only possible from the pSIS patches. MSC VEGFA production was increased by pSIS. Porcine SIS promoted an angiogenic gene profile in MSCs and an early cardiogenic profile in CPCs.

Conclusions: PTFE and pSIS allow for varying degrees of cell proliferation. pSIS elicits different phenotypical responses in MSCs as compared to CPCs which indicate that pSIS may modulate stem cell activation and proliferation. These findings highlight the differences in scaffold material strategies and suggest potential advantages of bioactive approaches.


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