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Perfusion Changes by Hyperspectral Imaging in a Burn Model
Oksana Babchenko BS1; Michael Chin MD1; Jorge Lujan MD1; Janice Lalikos MD1
1University of Massachusetts Medical School, Worcester, MA

Background: Early excision and skin grafting of full-thickness and deep-dermal burns is therapeutically and financially advantageous. However, differentiating between superficial and deep-dermal burns remains challenging. Superficial-dermal burns heal with conservative treatment; deep-dermal burns often require excision and skin grafting. Decision of surgical treatment is often delayed until burn depth is definitively identified. This study’s aim is to assess the ability of Hyperspectral Imaging (HSI) in differentiating burn depth.

Methods: Thermal injury of graded severity was generated on the dorsum of 76 hairless mice with a brass rod heated to 50, 60, 70, 80, or 90°C. Perfusion and oxygenation parameters of injured skin were measured with HSI, a non-invasive method of diffuse reflectance spectroscopy, at 24, 48 and 72 hours after wounding. Burn depth was measured histologically (n=44) at 72 hours.

Results: Three levels of burn depth were verified histologically: intermediate-dermal, deep-dermal, and full-thickness. At 24 hours post injury, total hemoglobin increased by 67% and 18% in intermediate and deep-dermal burns, respectively. In contrast, total hemoglobin decreased by 64% in full-thickness burns. Differences in deoxygenated and total hemoglobin between all groups were statistically significant (p


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